Coding

Part:BBa_K5321011:Design

Designed by: Yiyan Liao   Group: iGEM24_Peking   (2024-09-22)


nPPVp_mut


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 295
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 295
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 295
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 295
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Site-directed mutation should be performed.



Source

The original amino acid sequence of nPPVp was derived from Tina Fink et al. (2019). We reconstructed the corresponding nucleotide sequence and implemented site-directed mutagenesis.


References